Indian Institute of Technology Madras
 

Sample Requirements

For MALDI

Pure solids or solutions can be analysed. The sample should be vacuum compatible (no high vapour pressure material will be allowed). Literature on analysis details (in the case of known compounds) will be useful. On unknown/new samples, information on similar samples will be helpful. This reduces the time for optimizing the spectrometer performance.

Quantity should be of the order of 1 mg (for pure solids) or about 1 ml (in the case of solutions). For completely unknown samples, larger quantities are better as several trials may have to be done.

For TEM

(1) Dry powder or solution which evaporates on the TEM grid to form a dry film or (2) a thin film already prepared on the TEM grid or (3) microtomed biological specimen of < 70 nm thickness, etc. Nothing vacuum incompatible will be analysed. The typical column vacuum in our machine is < 1x10-5 pa. Samples which can cause strong beam induced damage should not be submitted which may harm the instrument. Sample must be submitted in advance as the grids will be prepared on the previous night (to reduce pumping time). Supporting information such as XRD / lower resolution TEM / SEM will be useful for the operator.

For Raman

Solids, solutions, liquids, thin films. Quantity should be of the order of 1 mg (for pure solids) or about 1 ml (in the case of solutions).

 
Nano Facilities